Instruction NTR
Job Description
This position requires a PhD degree and multiple years of postdoctoral training. The person will be responsible for carrying out and independently directing a research project that requires specialized knowledge and technical skills in biochemistry and molecular biology including but not limited to: 1) large volume cell culture including adaptation of cell lines to suspension culture, 2) isolation and purification of native protein complexes using carefully prepared buffers and ultracentrifugation, 3) preparation of in vitro reconstituted nucleosomes with human histone octamers and radio-labelled DNA, 4) precise calculation of ratios and timings to test nucleosome remodeling, ATPase, and dimer exchange activities, 5) pouring and running of Native-PAGE gels to confirm complex purity and/or assess remodeling or dimer exchange, 6) immune-affinity pulldowns of in vitro prepared DNA followed by library preparation and sequencing, 7) ability to work with an interdisciplinary team including bioinformatics analysts to interpret sequencing data, 8) drafting of manuscripts, preparation of figures, and presentation of work at national and international conferences, and 9) development of independent ideas and preliminary data for grant submission.